Therapeutic substance and process of manufacture



P atented July 16 1935,

UNITED V'STTATES PATIENT OFFICE THERAPEUTIC SUBSTANCE AND PROCESS OF'MANUFAOTUBE I Faw Yap Chuck, San Francisco, Calif. NoDrawing. Application Jilly 19, 1982, Serial I No.628,393 tOlalmafflllfl-SSX a This invention relates generally to therapeutic in the small intestine. Considering that the feedpreparations and to methods 01' compounding'the ing of milk mash can control cocoidiosis inthe same. The preparation is" particularly useful in caeca' and that only one out oisix knownspecies the control ci a certain avian disease commonly of ,coccidia afl'ect the caeca infthe case 01' the 5 termed coccidiosis: l chicken, themilkmash treatment is far from be 6 Coccidiosis is a fairly common diseasewhich ing satisfactory. Q I I attacks iowls' like chickens and turkeys,-'and ani- It is an object or my invention to provide "a malslikethe rabbit. It is caused-by the invasion coccidiosis remedy which'will kill the invading oi the intestinal; tract by an" organicparasite parasitic germs int ally without i a h 10 known as Eimera avian; These parasitic-germs tissues oi the patient. My remedy is character- 10 multiply at the expense of the cells oi! the izedby the fact that it canbeadministered to epithelium of the intestinal walls. and in some fowl or animals infected with any on or combicases the destruction of the tissues cause'ssever'e nation 0! all six species of coccldi'a that afiect the hemorrhage. There are'sixspecies oi the coo chicken,thati 'J Q lE-m f cidia known to affect chickens, two allfect turkeys, E/tenella, E. praecoz and E. necatrix, and will kill 15 quails and'pheasants, 'andoneandposgibly two the coccidia internally 'without any ill eifects afl'ect rabbits. Each species of coccidiahasa 119 11 thei wl- Th ame' slil h s been obiavorite area oi. the intestines to thrive. Hemt ll d w remedyih cocldlosis Cases ca l-l d orrhage and toxic substances given oil by the by Emeliaflfldis and E.melia aTimi i in r y 2o germs usually cause highmortality in chickens, E. disperse in quail and E. phasiani in pheasants. turkeys, pheasants, quai1s and rabbits. 1 It is a further object of the invention to provide Up to the present, the only treatment for thig a practical commercial method of manuiacturing disease meeting with any degree oi success, has my remedy. beenthe ieeding o! a mash containing 40% of dry r m dy onsists g l y Of a S ta le toxic skim or buttermilk. Chemicals and drug such alkaloid,-like nicotine, nicotinic acid or quinolinic 25 as'bismuth subnitra'te, zinc sulphocarbolate, poflj m o n d w h Dr n ria More tassium bichroinate, ibecca, hydrochloric acid and p c fi y e discovered at uch toxic in the like havebeen tried. and iound'ihadequate in s whichcan be termed pyrid Illi controlling the disease. Where "the iniection 'is i d i i s." when mn un ed with prointhe caeca, milk mash ieeding'is adequate, pair-v teinmaterial like albumin, have a powerful de- 30 ticularly where the mash contains 20% of lactose whve a on pon all known forms 01' coccldia by including 40% f d uk containing 50 7 .1 organisms, but will not-attack the tissue of the I tose,becausesuchaieed serves as a marked laxaintestines W hflfldetrlmentall? affect he tive. Thelaxative action causes voiding of the -P v r l have'beeh Secured y h 5' caeca content more oft n-than nor l, thus use of nicotine asthe main toxic ingredient, albringing'about rapld'elimination or coccidia from h h I v found that in order to f i e the caeca. This washing eflect togetherwith formatiflh 0 a Stable p d w en the the highly dig st bl nutrientsxfumshed; by t -nicotine and protein, it is desirable to employ milk along with the: increase-oi acidityin the tennioocidiand 1n orderto inoreesethe effec- 40 caeca', enables the fowl tocombat the disease suc-- 11 he m y in d ying undesired 40 cessfully. 1 Occasionally however the coccidia sms andat the same time to' minimize eliminated in the droppings'reiniect the iowl as toxicity 0! the nicotine pou d pflhh 10W! fast as it can pass them out. The result is an unother animal to which i administered, it

A controllable case, desirable'to iodize the nicotine. i

When the coccidia infection is located in the n o e spec ic di nt of y v t 45 upper and lower parts of the small intestine, my preparation consists of di-iodo-nicotine-tanwhere the coccidia E. acervulina and E. mitts for note or tetra-lode-nicotine-tannate, in combinainstance thrive best, treatment by milk mash tion with albumin. The effects of such nicotine or any other laxative material has no beneficial compounds on coccidia organisms has been effect. Chickens so infected when fed with milk shown by placing a specimen of the organism, 50 mesh will brighten tor a short time, but willagain either the oocyst or the sporulated cyst, on a become unhealthy soon after stopping the .milk microscopic slide and putting a solution-oi such mash feeding. Continuous feeding of 40% milk nicotine compounds on the side of the cover slip, mash tends to produce weak and soft intestinal allowing the solution to diffuse thru the slide.

56. tracts, and will not eliminate coccidia infection As soon as the iodo-nicotine solution comes in 6 contact with the coccidia organism, the outer coating of the organism begins to crack and the nucleus swells and eventually burst.

In treating infected chickens, the chickens are fed a grain mash containing suitable amounts of my preparation. A complete cure is generally effected in about four days. An overdosage of the remedy appearsto have no detrimental effects, and likewise it has been found that no harm is occasioned by continuing feeding of the mash after the chickens have been cured. Not only does treatment with my remedy cause disap nates,in.combination with albumin. These compounds taken separately have been found to act very slowly on coccidia, organism, but when the three are compounded together, the resulting material has a rapid destructive action. on the germs. The mixture produced from the three organic compoundsis not purely mechanical, but certain amounts of iodo-nicotine-tannate-glycinin ,are formed in the mixture. 1

The following is an outline of a practical process for the manufacture of di-iodo-nicotine-tannateglycinin. 50 lbs. of soybean (which is a source of glycinin) are soaked in water for 10 hours and then ground into a thick liquid milk. This is then filtered to remove coarse particles and fibrous material. The homogeneous filtrate is then heated to 195 deg. F. and divided into two equal portions.

To one portion of the soy bean milk add a solutionof 25 lbs. iodine in 30 lbs. 95% alcohol, while the milk is being constantly stirred. At this time glycinin, which'constitutes about to of the protein content of the soy bean milk, combines with part of the iodine; the starch in the milk combines with. another part of the iodine and the rest of the iodine is absorbed throughout the brown colloidal precipitate which is being formed in the process.

To the other portion of the soy bean milk there is added 38 lbs. of tannic acid pure) The tannic acid is added gradually and the milk continually stirred. A yellow precipitate of glycinin tannate is formed. About 1'7 lbs. of 95% pure nicotine are then added with continuous stirring. Adar-k .viscous mass of nicotine-glycinin-tannate, is formed.- i

The iodine proteincompound with absorbed iodine made from the first portion of the soy bean milk, is now mixed withthe nicotine compound made from the second portion of the milk, at about deg. A final product of di-iodonicotine-glycinin-tannate, is thus formed- This brownish black precipitate, being a colloid, has no clearcut physical characteristics. However the pure di-iodo-nicotine-tannate can be isolated, as

a distinct chemical compound forming definite needle crystals of Monoclinic form, which are reddish brown in color, having a melting point.

of C.

Tetra-lode nicotine tannate-glycinin, which has the same effect as. di-iodo-nicotine-tannateglyclnin, can be manufactured by the same process outlined above, except that twice the'amount of iodine is employed.

The above iodo-nicotine compounds are made more palatable by mixing the same with material like molasses. For example'the batch of di-iodonicotine-tannate-glycinin, obtained by the above outlined process, can be mixed with 80 lbs. of molasses. To facilitate mixing this material more evenly thru chicken feed, it can be rendered more bulky by mixing with 100 lbs. of ground wheat. 10 lbs. of this final mixture with 90 lbs. of a chicken mash, will give 0.05 lbs. of iodo-nicotineglycinin-tannate per lb. of mash. This dosage I preparation for this purpose, a dosage should include a suitable laxative, to properly clear the intestinal tract. Oneadvantage of my remedy when used as a cure for worms, is that it may be administered without a reduction in normal feed, and will not detrimentally affect the health and egg productivity of the chickens.

1. A therapeutic preparation for the destruction of intestinal parasites comprising di-iodo-nicotine-tannate-glycinin. V

2. A therapeutic preparation for'the destruction of intestinal parasites, comprising tetra-iodonicotine-tannate-glycinin 3. A process of manufacturing a therapeutic remedy comprising, adding tamiic acid and nicotine to a protein to form a precipitate of nicotine glycinin tannate,iodizing another batch of protein to form a precipitate, and then intermixing the two precipitates. i

- 4. A process of manufacturing a preparation of the character described, comprising forming a reaction product between iodine, tannic acid and protein, and thencausing the reacation product to further react with a material formed by reacting nicotine with a protein. v r 5. A preparationfor the destruction of intestinal' parasites, comprising an iodo-alkaloidtannate-protein. y

r 6. A preparation intestinal parasites, comprising an iodo-nlcotineuseful for the destruction of 

